PERK Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16373-50, BZ16373-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q9NZJ5

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: ICC/IF, WB, IHC-F, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 125 kDa; Observed MW: 125 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PERK in MCF-7 lysates using PERK antibody. The Lane on the right is blocked with the synthesized peptide., Immunofluorescence analysis of PERK in Hela using PERK antibody, Immunohistochemistry analysis of paraffin-embedded mouse lung tissue using PERK antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK

Immunogen: The antiserum was produced against synthesized peptide derived from human EIF2AK3. AA range:947-996

Conjugate: Unconjugated

Modification: Unmodified