NSF Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65677-50, BS65677-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P46459

Host: Rabbit

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,Zebrafish, Sheep,

Applications: WB

All Applications: WB=1:500-2000

Background: Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NSF Polyclonal Antibody detects endogenous levels of NSF protein.

Bioworld Molecular Weight: 83 kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Sample:
U937 (Human) Cell Lysate at 30 ug
Spleen (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-NSF at 1/1000 dilution

Alternative Name: N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor like protein; N ethylmaleimide sensitive fusion protein; N ethylmaleimide sensitive fusion protein; N-ethylmaleimide-sensitive fusion protein; NEM sensitive fusion protein; NEM sensitive fusion protein; NEM-sensitive fusion protein; NSF; NSF_HUMAN; SKD 2; SKD2; SKD2; Vesicle fusing ATPase; Vesicle fusing ATPase; Vesicle-fusing ATPase; Vesicular fusion protein NSF; Vesicular fusion protein NSF; Vesicular-fusion protein NSF.

Immunogen: KLH conjugated synthetic peptide derived from human NSF

Conjugate: Unconjugated

Modification: Unmodified