Cullin 4a Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90346-50, BS90346-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13619(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IHC FC

All Applications: WB:1:500-2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cullin 4a polyclonal antibody detects endogenous levels of Cullin 4a protein.

Bioworld Molecular Weight: 88 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cullin 4a on Hela cell lysates using anti-Cullin 4a antibody at 1/500 dilution., ICC staining Cullin 4a in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CUL-4A, CUL4A, 2810470J21Rik, AW495282, CUL 4A, CUL-4A, Cul4a, Cul4a protein, CUL4A, Cullin-4A, MGC36573, MGC64071,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified